Department of Infectious Diseases Prevention and Nosocomial Infections (ZP)

Personnel:

Head of the Department: Paweł Grzesiowski M.D, Ph.D
tel.: +48 22 851-52-05
Secretary: Beata Jasińska-Sawczyn
tel.: +48 22 851-52-05,
Iwona Łętowska M.D.Ph.D; tel. +48 22 841-40-71 ext.. 288
Jarosław Walory M.D Ph.D; tel. +48 22 841-40-71 ext. 206
Michał Karyński MD; tel. +48 22 841-40-71 ext. 206
Anna Olczak M.Sc.; tel. +48 22 851-52-05
Anna Ziółko M.Sc.; tel. +48 22 851-52-05
Małgorzata Turzyńska; tel. +48 22 841-40-71 ext. 288
Anna Bauc; tel. +48 22 841-40-71 ext. 208

Activities:

Coordination of National Surveillance Network of Antimicrobial Resistance and Antibiotic Consumption (OPTY) - a program accomplished as a part of National Health Program of the Ministry of Health. The network consists of over 60 hospitals from all over the country, collecting and analysing data about alert pathogens and antibiotic utilisation.

IMMU-LINE - telephone line for patients and medical staff, providing information on vaccination (tel. +48 0-601-838-939). Active since 1998.

Quality assurance:

  • Implementation of quality system in accordance with Polish Standard (PN-EN ISO/IEC 17025:2001)
  • Preparing procedures for laboratory accreditation process.

Educational activity

  • Courses, trainings, lectures, publications and methodical studies disseminating basic and practical knowledge in the field of microbiology diagnostics, rational antibiotic therapy, vaccination in clinical practice, clinical microbiology, antiseptics and analytical methods;
  • Professional courses for microbiologists and medical personnel;
  • Scientific seminars, conferences and symposia, e.g. Improvement in Infectious Diseases Medicine, International Scientific Workshops, School of Antibiotic Therapy;
  • Leading and coordinating of graduation thesis performance;
  • Practical summer training for students of Warsaw University, Medical School and Technical University;
  • Qualification and specialization courses for nurses and mid-wives in the field of epidemiological nursery;

Research:

Basic studies:

  • Antigens for vaccines production regarding emerging infections and new health threats;
  • Bacterial influence on human immune system in order to recognize infection diseases pathogenesis among different patients' groups and different experimental models;
  • Effectiveness of vaccination schedules;

Application studies:

  • Immunization assessment by serological monitoring of antibodies in order to identify people who are not immunized. Outcomes of the studies are used for modifying the general and  recommended programs of preventive vaccinations.
  • Epidemiological studies regarding new vaccines introduced in National Vaccination Program and vaccines recommended for risk groups.
  • Preparing biological standards and new bacterial strains for Polish vaccine and blood products industry.
  • Cooperation in creating:
    • hospital formularies:
    • hospital infections evaluation programs;
    • antibiotic policy.

Study projects

Main projects since 2000:

  • National Health Programs: OPTY, ALERT, National Program of Prudent Use of Antibiotics.
  • International Programmes: European Antimicrobila Resistance Surveillance System (EARSS), European Surveillance of Antibiotic Consumption (ESAC), Self-medication with Antibiotics (SAR), Validation of serologic methods (BSP 034, EDQM, Council of Europe).

Cooperation:

  • Ministry of Health, health care centres, epidemiology and veterinary services.
  • European Directory for Quality of Medicines (EDQM), European Pharmacopoeia Commission.
  • Universities:
    • Biotechnology Department of Warsaw School of Technology;
    • Faculty of Biology of Warsaw University,
    • Faculty of Pharmacy, Medical University of Warsaw.

Services:

  • Expertise in the field of hospital infections control.
  • Expertise in the field of preventive vaccinations in clinical practice.
  • Recommendations and rules of rational use of antibiotics.
  • Infectious diseases diagnostics.
  • Epidemiological investigations.
  • Immuno-service.

Equipment:

  • Bio-Logic DuoFlow system(Bio Rad) for semi-preparative chromatography, flow rate to 1000psi, UV/VIS detector, conductivity monitor;
  • Bio Focus capillary electrophoresis (Bio Rad);
  • Flow cytometer BD FACSCalibur System (Becton Dickinson);
  • Microplate spectrophotometer, spectral scan, Power Wave X (Bio-Tec Instruments Ins.), UV-VIS: 200-999nm (enzymatic kinetics, whole ELISA range);
  • Plate fluorometer and luminometer Fluoroscan Ascent FL (Labsystem), FITC filters (485/538nm), (544/590nm);
  • Two-dimensional electrophoresis system; Pharmacia;
  • Liophilisator alfa 1-4 (Christ);
  • Fermentor (for volumes to 10l) Microferm Fermentor (New Brunswick Scientific Co. Inc.);
  • Incubator CO2 - Assab Green Line;
  • Centrifuge up to 2000 rpm, 6x 500ml Mistral 6L (MSE);
  • Incubators, freezers(-75°C also), shakers, baths, laminar boxes, pH-meters, centrifuges, scales, autoclaves, driers, water purifying system (destilation, deionisation);

Main publications since 2000

  • Walory J., Grzesiowski P., Hryniewicz W.
    Comparison of four serological methods for detection of diphtheria antitoxin antibody.
    Journal of Immunological Methods. 2000, 245(1-2): 55-65.
  • Krcmery V., Jeljaszewicz J., Grzesiowski P., Hryniewicz W., i wsp.
    National and local antibiotic policies in Central and Eastern Europe.
    J. Chemother. 2000 Dec;12(6):471-4.
  • Walory J., Grzesiowski P, Hryniewicz W
    The prevalence of diphtheria immunity in healthy population in Poland.
    Epidemiology & Infection, 2001,126 (2): 225-230.
  • Hryniewicz W., Grzesiowski P., Ozorowski T.
    Hospital infection control in Poland.
    J Hosp Infect. 2001 Oct;49(2): 94-8.
  • Allerberger F., Ayliffe G., Bassetti M., Braveny I., Bucher A., Damani N., Daschner F., Dettenkofer M., Ezpeleta C., Gastmeier P., Geffers C., Giamarellou H., Goldman D., Grzesiowski P., i wsp.
    Routine surface disinfection in health care facilities: should we do it?
    Am J Infect Control. 2002 Aug;30(5): 318-9.
  • Fiett J., Łętowska I., Gniadkowski M., Hryniewicz W.
    The new strategy for allele identification of the genes coding for pertussis toxin subunit S1 (ptx S1) and pertactin (prn) in Bordetella pertussis.
    J. Microbiol. Methods. 2003, Dec; 55(3): 651-66.
  • Łętowska I., Hryniewicz W.
    Epidemiology and characterization of Bordetella parapertussis strains isolated between 1995 and 2002 in and around Warsaw, Poland.
    Eur. J. Clin. Microbiol. Infect. Dis. 2004 Jun; 23(6): 499-501.
  • Sulikowska A., Grzesiowski P., Sadowy E., Fiett J., Hryniewicz W.
    Characteristics of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis isolated from the nasopharynges of asymptomatic children and molecular analysis of S. pneumoniae and H. influenzae strain replacement in the nasopharynx..
    J. Clin. Microbiol. 2004 Sep; 42(9): 3942-9.
  • Cizman M., Beovic B., Krcmery V., Barsic B., Tamm E., Ludwig E., Pelemis M., Karovski K., Grzesiowski P., i wsp.
    Antibiotic policies in Central Eastern Europe.
    Int. J. Antimicrob. Agents. 2004 Sep; 24(3): 199-204.
  • Fiszer U., Tomik B., Grzesiowski P. Krygowska-Wajs A., Walory J. and others
    The antibodies against Bordetella pertussis in sera of patients with Parkinson's disease and other non-neurological diseases.
    Acta Neurol. Scand. 2004 Aug; 110(2): 113-7.