Department of Cell Biology

Staff:

Head: Zdzisław Chilmonczyk, Ph.D., D.Sc., Professor
phone: 022 851 43 69, 022 851 44 96
fax: 022 840 63 30,  e-mail:

Secretary: Barbara Najer
phone:   +48 22 841-36-89 ext. 424
+48 22 841-36-83 ext. 424
+48 22 840-34-60 ext. 424


Flow Cytometry Laboratory
Head: Janusz S. Skierski, M.D., Ph.D., 841-06-42 ext. 175 or 412;  e-mail:
Jarosław Walory, Ph.D.
Sylwia Chełstowska, M.Sc.
Mirosława Koronkiewicz, M. Sc.
Sylwia Szafraniec, M.D.

Confocal Microscopy Laboratory
Head:Teresa Kasprzycka-Guttman, Ph.D., D.Sc., Professor; phone: 851 43 81 ext. 324,  e-mail:
Irena Misiewicz, Ph.D.
Katarzyna Skupińska, Ph.D.

Activities:

Quality control of medicinal products:

Carrying out the Institute's tasks concerned with medicines quality assurance and safety (medicines and biocidal products, registered and evaluated products). Evaluation of drug registration files. Expert services for The Office for Registration of Medicinal Products, Medical Devices and Biocidal Products

Educational activity:

  • Professional trainings for pharmacy, biology, chemistry and biotechnology students
  • Training in the fields of: flow cytometry and fluorescent microscopy, cell maintenance, preparation of microscopic slides especially for purpose of confocal microscopy (selection of dyes, microscopic slides), observation with confocal microscopy and image analysis
  • Conducting and supervising M.Sc. and Ph.D.thesis

Research:

  • Drug-receptor interaction
  • Mechanisms of drug action
  • Molecular biochemistry and biology
  • Biology of neoplastic cells
  • Synthesis of biologically active compounds
  • Evaluation of anticancer and chemopreventive drugs
  • Cytotoxic activity
  • Enzymatic activity evaluation
  • Mechanisms of apoptosis and cellular cycle parameter chamnges

The following techniques are used:

  • Flow cytometry
  • Microscopy -  fluorescence, phase contrast and Nomarsky contrast
  • molecular biology techniques.
  • Western Blotting
  • Various neoplastic and non-neoplastic cell lines are used to perform in vitro tests. No tests on animals are performed.

Grants

  • Research project PO5F 019 27: Determination of chemopreventive properties and mechanisms of activity of selected sulforaphane analogues
  • Research Project 3 T09A 032 29: Examination of some serotonin transporter inhibitors interactions with human serotonin transporter model with the aid of molecular modeling

Participation in NIL scientific projects in 2008

  • 1.18. Chiral chromatography of medicines and pharmacologically avtive substances evaluation
  • 1.58..Determination of medicines and their metabolites environmental levels
  • 2.14. Molecular base of drug- receptor interaction
  • 2.20. QSAR of sulforaphane analogues
  • 3.12. Molecular bases of sulforaphane and its analogues chemopreventive activity
  • 3.13. Evaluation of Selol cytotoxic activity towrds tumor cell lines
  • 3.14.Isothiocyanates as PAHs metabolizm inhibitors - mechanizm of activity
  • 6.13. Evaluation of synergistic activity of CK2 kinaze inhibitors and nucleoside derivatives on human tumor cell lines
  • 7.51. Evaluation of glutamatergic receptor inhibitors influence on drug-induced apoptosis
  • 7.52. Examination of sulforaphane farmakokinetics and tissue distribution

Cooperation:

  • Medical University, Warsaw
  • Warsaw Agricultural University (SGGW)
  • NENCKI Institute of Experimental Biology PAS
  • Medical Research Center, PAS
  • Institute of Psychiatry and Neurology in Warsaw
  • Institute of Oncology in Warsaw
  • Institute of Hematology, Warsaw
  • Cancer Research Institute at The New York Medical College, Hawthorne, NY, USA
  • MD Anderson Cancer Center, Houston, TX, USA
  • Department of Medical Biology, Tromso University, Tromso, Norway
  • Pomeranian Medical University
  • Department of Chemistry, Warsaw University
  • Military University of Technology

Services:

  • Determination of DNA content in the cells, together with the determination of cell cycle parameters in clinical and experimental samples
  • Determination of apoptotic cell fraction, using the Annexin V/Pi, PARP cleavage and TUNEL methods
  • Various viability tests
  • Determination of LD50 value using cell lines in culture
  • Immunophenotyping of human blood lymphocytes
  • Cell morphology using fluorescence microscopy, phase-contrast and Nomarsky contrast techniques
  • High resolution digital microphotography (5 Mpixel)
  • Microscopic image recording
  • Image analysis
  • Cytotoxic and chemopreventive properties of natural and synthetic substances
  • Drug crystal shape.analysis
  • Influence of shampoo, conditioners and hair dyes on hair quality
  • Cytotoxity of natural and synthetic substances on multi-drug resistant cells
  • Tooth surface analysis (enamel quality, the structure of tooth surface and quality of filling)

Equipment:

  • FACS Vantage flow cytometer. Excitation wavelength: UV (351 nm), blue (488 nm), red (633 nm). Analysis channels: blue (424 nm), green (530 nm), orange (575 nm), red (630 nm), far red (>660 nm)
  • FACS Calibur flow cytometer. Blue argon laser (488 nm) as an excitation light source, fluorescence channels green, orange and red
  • Olympus BX60 fluorescence microscope with full optics and DP50 digital camera (cooled CCD, 5 MPixels)
  • Shimadzu UV-1700 PharmaSpec spectrophotometer (190 - 1100 nm)
  • PowerWave XS Biotek microplate reader
  • Tissue culture and cell bank equipment (laminar flow, CO2 incubator, liquid nitrogen tanks (dewars, stem aotoclave)
  • Inverted microscope Olympus IX70 (with epifluorescence, phase contrast, Nomarski contrast)
  • Confocal Microscope Olympus IX70 with scanning system Fluoview 500 equipped with 4 lasers: UV (351 nm), Ar (488 nm), He-Ne (543 nm) and He-Ne (633 nm). 4 fluorescence detection channels and transmitted light channel. Image analysis software: Fluoroview500, Imaris (Bitplane)
  • WesternBlot set: Bio-Rad Mini-Protean 3
  • Centrifuge MPW-350R (MPW)

Selected papers published since 2004

  1. I. Misiewicz., K. Skupińska, T. Kasprzycka-Guttman.
    "Sulforaphane-mediated induction of phase 2 detoxifying enzyme NAD(P)H: quinone reductase and apoptosis in human lymphoblastoid cells"
    Acta Biochim. Pol. 51, 711-721 (2004).
  2. Z. Chilmonczyk, M. Mazgajska, E. Chojnicka-Wójcik, E. Tatarczyńska, A. Kłodziska, M. Ulman.
    Synthesis, pharmacological activity and SAR of New 1,4-disubstituted piperazine derivatives with hypnotic-sedative activity.
    Pol. J. Chem. 78, 1027-1037 (2004).
  3. I. Misiewicz, A. Kozar, K. Skupinska, E. Kowalska, J. Lubinski, T. Kasprzycka-Guttman.
    Inhibition of cell cycle and induction of apoptosis by sulforaphane in cell lines carrying various inherited BRCA1 mutations.
    Oncol Rep. 13, 659-65 (2005).
  4. K. Stachnik, P. Grieb, J. Skierski:
    Cytotoxic effects of cladribine and tezacytabine toward HL-60.
    Acta Biochim. Pol. 52, 561-565 (2005).
  5. K. Skupinska, M. Zylm, I. Misiewicz, T. Kasprzycka-Guttman.
    Interaction of anthracene and its oxidative derivatives with human serum albumin.
    Acta Biochim. Pol. 53, 101-112 (2006).
  6. I. Misiewicz, A. Kozar, K. Skupinska, E. Kowalska, J. Lubinski, T. Kasprzycka-Guttman.
    Inhibition of Cell Cycle and Induction of Apoptosis by 2-Oxoheksyl Isothiocyanate and Alyssin in Cell Lines Carrying Various Inherited BRCA1 Mutations.
    Drug Dev. Res. 65, 84-92 (2005).
  7. B. M. Gruber, E. L. Anuszewska, I. Bubko, T. Kasprzycka-Guttman, I. Misiewicz, K. Skupińska, W. Priebe, I. Fokt.
    Constitutive activation of transcription factor NFκB as a possible marker of sensitivity of neoplastic cells to anthracyclines.
    Acta Pol. Pharm. Drug Res. 62, 17-23 (2005).
  8. J. Iskra-Jopa, K. Giołembiowska, A. Dziubina, M. Cybulski, B. Duszyńska, Z. Chilmonczyk.
    In vivo effects of the 1,2,4-piperazine derivatives MM5 and MC1, putative 5-HT agonists, on dopamine and serotonin release in rat prefrontal cortex.
    J. Pharm. Pharmacol. 57, 205-211 (2005).
  9. Z. Chilmonczyk, H. Ksycińska, M. Lisowska-KuĽmicz, A. Jończyk, M. Mazgajska, M. Jarończyk, A. Strzelczyk, H. Y. Aboul-Enein.
    Structure-enantioselectivity relationship of hypnotic-sedative 1,4-disubstituted piperazine derivatives on cellulose tris(4-methylbenzoate) chiral stationary phase.
    Anal. Chim. Acta. 536, 7-13 (2005).
  10. T. Magdziarz, B. Łozowicka, R. Gieleciak, A. B±k, J. Polański, Pogański. Chilmonczyk.
    3D QSAR study of hypolipidemic asarones by comparative molecular surface analysis.
    Bioorg. Med. Chem. 14, 1630-1643 (2006)..
  11. D±browska M., Hendrikx PJ., Skierski J., Malinowska M., Bertino JR., Rode W.:
    EGFP fluorescence as an indicator of cancer cells response to methotrexate.
    Eur. J. Pharmacol. 555(2-3), 93-99 (2007).
  12. Issat T., Nowis D., Legat M., Makowski M., Klejman MP., Urbański J., Skierski J., Koronkiewicz M., Stoklosa T., Brzezinska A., Bil J., Gietka J., Jakóbisiak M., Golab J.:
    Potentiated antitumor effects of the combination treatment with statins and pamidronate in vitro and in vivo.
    Int. J. Oncology 30(6), 1413-1425 (2007).
  13. Skupinska K, Misiewicz I, Kasprzycka-Guttman T:
    A comparison of the concentration-effect relationships of PAHs on CYP1A induction in HepG2 and Mcf7 cells.
    Archives of Toxicology 81(3), 183-200 (2007).
  14. Krzemiński P, Misiewicz I, Pomorski P, Kasprzycka-Guttman T, Barańska J.
    Mitochondrial localization of P2Y1, P2Y2 and P2Y12 receptors in rat astrocytes and glioma C6 cells.
    Brain Res. Bull. 71(6), 587-592 (2007).
  15. Misiewicz I, Skupinska K, Kasprzycka-Guttman T.
    Differential response of human healthy lymphoblastoid and CCRF-SB leukemia cells to sulforaphane and its two analogues: 2-oxohexyl isothiocyanate and alyssin.
    Pharmacol. Reports 59(1), 80-87 (2007).
  16. Treszczanowicz T., Kasprzycka-Guttman T., Trzeszczanowicz A.,
    J. Solubility of β-carotene in binary solvents formed by some hydrocarbons with 2,5,8-trioxane, 2-propanone and cyclohexanone.
    J. Chem. Eng. Data 52, 261-264 (2007).
  17. M. Ulman, K. Bielawska, B. Łozowicka, M. Heimann, J. Kesselmaier, G. Scebeske, K. S. Katryński, Z. Chilmonczyk.
    Determination of volatile organic compounds (VOCs) in the atmosphere over Central Siberian forest and southern part of European Taiga in Russia.
    Cem. Anal (Warsaw) 52, 435-451 (2007).
  18. M. Ulman, Z. Chilmonczyk.
    Volatile organic compounds - components, sources, determination. A review.
    Chem. Anal. (warsaw) 52, 173-200 (2007).
  19. M. Koronkiewicz, M. Szkop, Z. Kazimierczuk, M. Bretner, J. Skierski.
    The cytostatic and cytotoxic effects of protein kinase CK2 inhibitors on the neoplastic cells in vitro.
    Acta Biochimica Polonica 54S, 39 (2007).
  20. Z. Chilmonczyk.
    "Od substancji prostych do życia. ¦wiat RNA - pocz±tki życia na Ziemi."
    Biblioteka Wiadomo¶ci Chemicznych, Wrocław 2007, ISBN 83-229-2084-9.