Department of Pharmacology (FA)

Personnel and structure:

Head of the Department: Zenon Jastrzębski, Ph.D., D.V.M.
phone: +48 22 841 06 42 ext. 124
phone/fax:  +48 22 841 56 20
room: 410

Secretary: Anna Domżała
phone: +48 22 841 06 42 ext. 410
phone/fax:  +48 22 841 56 20
room: 410
e-mail:  sekretariatfa

Toxicological Lab (FA/T)
Head: Anna Jędrych, Ph.D; phone: +48 22 841 06 42 ext. 416
Mirosława Duras - Grabalska; phone: +48 22 841 06 42 ext. 413
Renata Krzysztofik; phone. +48 22 841 06 42 ext. 413

Hemostasis and Hemodynamics Lab. (FA/H)
Head: Małgorzata Remiszewska, Ph.D; phone: +48 22 841 06 42 ext. 416
Sylwia Flis Ph.D; phone: +48 22 841-06-42 ext. 419
Katarzyna Więckowska; phone: +48 22 841 06 42 ext. 418

Pharmaceutical Medicine Lab. (FA/M)
Head: Paweł Januszewicz, Professor; phone: +48 22 841 29 40 ext.157

Experimental Animal Lab. (FA/Z)
Head: Zenon Jastrzębski Ph.D; phone: +48 22 841 06 42 ext. 124
Tomasz Piotrowski; phone: +48 22 841 06 42 ext. 422
Michał Sawczyn; phone: +48 22 841 06 42 ext. 422

Activities:

Quality control of medicinal products:

  • Pharmacopoeal biological tests: pyrogens, abnormal toxicity, depressor substances, bacterial endotoxins (tast LAL)
  • Single dose toxicity studies
  • Non-clinical local tolerance testing and evaluation of sensitizing potential
  • Particle size analysis by laser light diffraction
  • Pharmacometric analyzes
    • hypnotic activity
    • anesthetic and analgesic activity
    • anticonvulsant activity
    • coagulation tests
  • Complex physico-chemical analysis of medicinal products and disinfectants
  • Evaluation of dossiers in the process of registration of medicinal products: injections (lipids, anticoagulants, infusion liquids), antiseptics, antineoplastics, immunomodulators and selected dental materials
  • Pharmaceutical equivalence of medicinal products
  • Bioequivalence of medicinal products

Quality assurance:

  • Implementation of quality system in accordance with PN-EN ISO/IEC 17025:2005
  • Elaboration of quality system documents (Standard Operation Procedures, instructions, log-books for the equipment)
  • Participation in Proficiency Testing Studies organized by EDQM
  • Participation in Inter-Laboratory Performance Qualification for LAL Test organized by Associates of Cape Cod, Inc.

Research:

  • The role of epigenetic modifications in the mechanism of anticancer drug action
  • Methylation of genes determining cellular function and its influence on the effectiveness of anticancer therapy
  • Chemopreventive activity of drugs and their antiangiogenic potential
  • Studies on synergism and antagonism between drugs
  • Local tolerance of drugs and medical devices in different pharmaceutical formulations
  • Antiatherogenic activity of various natural drugs and their preventive potential in cardiovascular diseases. Studies on experimental hiperlipidemia and atherosclerosis
  • Bioavailability of drugs
  • Pharmacoeconomics of some drugs and vaccines
  • Theoretical aspects of clinical tests: efficacy and toxicology issues

Educational activity

The department runs the following trainings:
  • Usage and performance of LAL test in quality control of parenteral drugs
  • Cell culture methods and cytotoxicity testing
  • Summer training for students from Medical University and Agricultural University

Cooperation:

  • Polish Pharmacopoea, Antibiotics and Biological Methods Commission
  • Department of Medical Chemistry and Natural Products, School of Pharmacy, The Hebrew University of Jerusalem, Israel
  • Institute of Cardiology, Kaplan University Medical Centre, Rehovot, Israel
  • Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Warsaw
  • Department of Pathological Anatomy, Center of Biostructure Research, Medical University of Warsaw
  • Laboratory of Mutagenesis and DNA Repair, Institute of Biochemistry and Biophysics PAS, Warsaw
  • Department of Microbioanalytics, Warsaw University of Technology

Services:

  • Toxicity studies in vivo (single dose toxicity and pharmacopoeial safety tests) and in vitro (MTT and NRU assays)
  • Non-clinical local tolerance testing after various routs of drug administration including microscopic analysis of histopathologic changes at the site of drug application
  • Evaluation of sensitizing potential of medicinal products (delayed-type hypersensitivity)
  • Testing of pharmacological activity of final medicinal products:
    • analgesics
    • hypnotics
    • local anaesthetics
    • drugs acting on cardiovascular system
    • anticoagulants
  • Mechanical contaminations in solutions and gases and determination of particle size distribution
  • Quality assessment of some pharmaceutical products (solutions, lipids for parenteral administration, antiseptics, etc.) as a part of medicines control
  • LAL test and rabbit pyrogen test training and consulting
  • Evaluation of medicinal products for registration and other purposes

Equipment:

  • HPLC system Shimadzu with UV/VIS and spectrofluorometric detectors
  • HPLC system Dionex with UV/VIS, DAD, RF and RI detectors
  • spectrophotometer UV/VIS
  • microplate spectrophotometer
  • the sets of horizontal and vertical gel electrophoresis
  • cabinet with laminar air-flow
  • incubators
  • thermoblocks
  • termocycler
  • system for Image Analysis
  • liophilizator
  • laser analyzer of particle size
  • fluid autocounter
  • potentiometric titrator
  • ELx808 Incubating Reader for automated measurement of endotoxin content
  • muffle furnace
  • automated computerized temperature recorder for pyrogen testing in rabbits
  • thermometer calibration system
  • image analysis system MultiScane Base
  • hot air ovens, shakers, centrifuges, pH meters, water bathes, microscopes, water purification systems

Publications (2007-2010)

  1. Flis S, Flis K, Gnyszka A, Spławiński A.
    MS275 enhances cytotoxicity induced by 5-fluorouracil in the colorectal cancer cells.
    Eur J Pharmacol 2010: 627: 26 32.
  2. Flis S, Gnyszka A, Spławiński A.
    HDAC inhibitors, MS275 and SBHA, enhance cytotoxicity induced by oxaliplatin in the colorectal cancer cell lines.
    BBRC 2009: 336-341.
  3. Flis S, Gnyszka A, Misiewicz-Krzemińska I, Spławiński A.
    Decytabine enhances cytotoxicity induced by oxaliplatin and 5 fluorouracil in the colorectal cancer cell line Colo 205.
    Cancer Cell Inter. 2009: 9: 10.
  4. Gorinstein S, Leontowicz H, Leontowicz M, Jastrzębski Z, Najman K, Tashma Z, Katrich E, Heo BG, Cho JY, Park YJ, Trakhtenberg S.
    The influence of raw and processed garlic and onions on plasma classical and non-classical atherosclerosis indices: investigations in vitro and in vivo.
    Phytother Res. 2010: 24:706-14.
  5. Flis S, Spławiński J.
    Inhibitory effects of 5 fluorouracil and oxaliplatin on human colorectal cancer cells survival are synergistically enhanced by sulindac sulfide.
    Anticancer Res. 2009: 29: 435 442.
  6. Leontowicz H., Leontowicz M., Haruenkit R., Poovarodom S., Jastrzębski Z., Drzewiecki J., Ayala A.L., Jesion I., Trakhtenberg S., Gorinstein S.
    Durian (Durio zibethinus Murr.) cultivars as nutritional supplementation to rat`s diets.
    Food Chem Toxicol 2008: 46, 581-589.
  7. Gorinstein S., Leontowicz M., Leontowicz H., Namieśnik J., Jastrzębski Z., Drzewiecki J., Park, Y.-S., Ham K.-S., Heo B.-G, Trakhtenberg, S.
    Influence of mussels (Mutilus galloprovincialis ) from polluted and non-polluted areas on some atherosclerosis indices in rats fed cholesterol.
    Food Chem 2008: 111, 381-386.
  8. Gorinstein S., Jastrzębski Z., Leontowicz H., Leontowicz M., Namiesnik J., Najman K., Park Y-S, Heo B-G, Cho J-Y, Bae J-H.
    Comparative control of the bioactivity of some frequently consumed vegetables subjected to different processing conditions.
    Food Control, 2009: 20, 407-413.
  9. Flis S, Flis K, Spławiński J.
    Epigenetic modifications and cancer.
    Nowotwory Journal of Oncology 2007: 57: 427-434.
  10. Gorinstein S., Jastrzębski Z., Namieśnik J., Leontowicz H., Leontowicz M., Trakhtenberg S.
    The atherosclerotic heart disease and protecting properties of garlic: contemporary data.
    Mol Nutr Food Res 2007: 51, 1365-1381.
  11. Gorinstein Sh., Caspi A., Libman I., Leontowicz H., Leontowicz M., Tashma Z., Katrich E., Jastrzębski Z., Trakhtenberg S.
    Bioactivity of beer and its influence on human metabolism.
    Int J Food Sci Nutr 2007: 58, 94-107.
  12. Jastrzębski Z., Gorinstein Sh., Czyżewska-Szafran H., Leontowicz H., Leontowicz M., Trakhtenberg S., Remiszewska M.
    The effect of short-term lyophilized beer on established hypertension in rats.
    Food Chem Toxicol 2007: 45, 296-302.
  13. Jastrzębski Z., Leontowicz H., Leontowicz M., Namieśnik J., Zachwieja Z., Barton H., Pawelzik E., Arancibia-Avila P., Toledo F., Gorinstein S.
    The bioactivity of processed garlic (Alium sativum) L.) as shown in vitro and in vivo studies on rats.
    Food Chem Toxicol. 2007: 45, 1626-1633.
  14. Jastrzębski Z., Napiórkowska L., Pykało R.
    The histopathological evaluation of the local tolerance of alendronate containing medicines.
    Endokrynol Pol 2007: 58, 11-17.
  15. Jastrzębski Z., Tashma Z., Katrich El., Gorinstein S.
    Biochemical characteristics of the herb mixture Prolipid as a plant food and medical remedy.
    Plant Foods Human Nutr 2007: 62, 145- 150.