Department of Pharmacology (FA)

Personnel and structure:

Head of the Department: Zenon Jastrzębski, Ph.D.
phone: +48 22 841 06 42 ext. 124
phone/fax:  +48 22 841 56 20
room: 410

Secretary: Anna Domżała
phone: +48 22 841 06 42 ext. 410
phone/fax:  +48 22 841 56 20
room: 410
e-mail:  sekretariatfa

Toxicological Lab
Head: Anna Jędrych, Ph.D; phone: +48 22 841 06 42 ext. 416; annajedrych
Mirosława Duras - Grabalska; phone: +48 22 841 06 42 ext. 413; mirkad
Renata Krzysztofik; phone. +48 22 841 06 42 ext. 413

Blood and Circulation Lab
Head: Małgorzata Remiszewska, Ph.D; phone: +48 22 841 06 42 ext. 416; kora13
Elżbieta Zub; phone: +48 22 841 06 42 ext. 418
Katarzyna Więckowska; phone: +48 22 841 06 42 ext. 418
Jan Chocianowski; phone: +48 22 841 06 42 ext. 424

Bioavailability Lab
Head: Jacek Spławiński, Professor; phone: +48 22 841 06 42 ext. 422; splawinski
Sylwia Flis, Ph.D; +48 22 841 06 42 ext. 419
Agnieszka Jagodzińska, M.Sc; +48 22 841 06 42 ext. 419

Pharmacoeconomic Lab
Head: Jacek Spławiński, Professor; +48 22 841 06 42 ext..422

Pediatric Pharmacotherapy Lab
Head: Paweł Januszewicz, Professor; phone: +48 22 841 29 40 ext.157

Activities:

Quality control of medicinal products:

  • Pharmacopoeal biological tests: pyrogens, abnormal toxicity, depressor substances, bacterial endotoxins (tast LAL)
  • Single dose toxicity studies
  • Non-clinical local tolerance testing and evaluation of sensitizing potential
  • Particle size analysis by laser light diffraction
  • Pharmacometric analyzes
    • hypnotic activity
    • anesthetic and analgesic activity
    • anticonvulsant activity
    • coagulation tests
  • Complex physico-chemical analysis of medicinal products and disinfectants
  • Evaluation of dossiers in the process of registration of medicinal products: injections (lipids, anticoagulants, infusion liquids), antiseptics, antineoplastics, immunomodulators and selected dental materials
  • Pharmaceutical equivalence of medicinal products
  • Bioequivalence of medicinal products

Quality assurance:

  • Implementation of quality system in accordance with PN-EN ISO/IEC 17025:2005
  • Elaboration of quality system documents (Standard Operation Procedures, instructions, log-books for the equipment)
  • Participation in Proficiency Testing Studies organized by EDQM
  • Participation in Inter-Laboratory Performance Qualification for LAL Test organized by Associates of Cape Cod, Inc.

Educational activity

The department runs the following trainings:
  • Usage and performance of LAL test in quality control of parenteral drugs
  • Cell culture methods and cytotoxicity testing
  • Pharmacoeconomics
  • Evidence Based Medicine
  • Summer training for students from Medical University and Agricultural University

Research:

  • The role of epigenetic modifications in the mechanism of anticancer drug action
  • Methylation of genes determining cellular function and its influence on the effectiveness of anticancer therapy
  • Chemopreventive activity of drugs and their antiangiogenic potential
  • Interaction between selected cytostatics studied in cancer cell lines in vitro
  • Studies on synergism and antagonism between drugs
  • Local tolerance of drugs in different pharmaceutical formulations
  • Antiatherogenic activity of various natural drugs and their preventive potential in cardiovascular diseases. Studies on experimental hiperlipidemia and atherosclerosis
  • Bioavailability of drugs
  • Pharmacoeconomics of some drugs and vaccines
  • Theoretical aspects of clinical tests: efficacy and toxicology issues

Cooperation:

  • Polish Pharmacopoea, Antibiotics and Biological Methods Commission
  • Department of Medical Chemistry and Natural Products, School of Pharmacy, The Hebrew University of Jerusalem, Israel
  • Institute of Cardiology, Kaplan University Medical Centre, Rehovot, Israel
  • Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Warsaw
  • Department of Pathological Anatomy, Center of Biostructure Research, Medical University of Warsaw

Services:

  • Toxicity studies (single dose toxicity and pharmacopoeial safety tests: abnormal toxicity, depressor substances, bacterial endotoxins, pyrogens)
  • Non-clinical local tolerance testing after different routs of drug administration including microscopic analysis of histopathologic changes at the site of drug application
  • Evaluation of sensitizing potential of medicinal products (delayed-type hypersensitivity)
  • Testing of pharmacological activity of final medicinal products:
    • analgesics
    • hypnotics
    • local anaesthetics
    • drugs acting on cardiovascular system
    • anticoagulants
  • Mechanical contaminations in solutions and gases and determination of particle size distribution
  • Quality assessment of some pharmaceutical products (solutions, lipids for parenteral administration, antiseptics, etc.) as a part of medicines control
  • LAL test and rabbit pyrogen test training and consulting
  • Evaluation of medicinal products for registration and other purposes
  • Pharmacoeconomic analyzes

Equipment:

  • HPLC- Shimadzu with UV and spectrofluorometric detectors
  • spectrophotometer UV-VIS
  • PCR, RFLP, RT-PCR
    • poliacrylamid and agarose electrophoresis
    • thermocycler
    • thermoblock (4o C - 100o C)
  • protein electrophoresis
  • liophilizator
  • laminar flow cabinet
  • laser analyzer of particle size
  • fluid autocounter, Malvern
  • potentiometric titrator
  • ELx808 Incubating Reader for automated measurement of endotoxin.content
  • muffle furnace
  • automated computerized temperature recorder for pyrogen testing in rabbits
  • thermometer calibration system
  • image analysis system MultiScane Base
  • hot air ovens, shakers, centrifuges, pH meters, water bathes, microscopes, water purification systems

Main publications since 2003

  • Elwich-Flis S., Sołtysiak-Pawluczuk D., Spławiński J.:
    Antiangiogenic and apoptotic effects of metabolites of sulindac on chick embryo chorioallantoic membrane.
    Hybridoma Hybridom 22, 56-60 (2003).
  • Flis S., Flis K., Spławiński J.:
    Modyfikacje epigenetyczne w nowotworach. Nowotwory.
    Journal of Oncology 57, 67-73 (2007).
  • Flis S., Sołtysiak-Pawluczuk D., Jędrnych A., Jastrzębski Z., Remiszewska M., Spławiński J.:
    Antiangiogenic effect of sulindac sulfide could be secondary to induction of apoptosis and cell cycle arrest.
    Anticancer Res. 26, 3033-3041 (2006).
  • Gorinstein S., Jastrzębski Z., Namieśnik J., Leontowicz H., Leontowicz M., Trakhtenberg S.
    The atherosclerotic heart disease and protecting properties of garlic: contemporary data.
    Mol Nutr Food Res 51, 1365-1381 (2007).
  • Gorinstein Sh., Caspi A., Libman I., Leontowicz H., Leontowicz M., Tashma Z., Katrich E., Jastrzębski Z., Trakhtenberg S.
    Bioactivity of beer and its influence on human metabolism.
    Int J Food Sci Nutr 58, 94-107 (2007).
  • Jastrzębski Z., Gorinstein Sh., Czyżewska-Szafran H., Leontowicz H., Leontowicz M., Trakhtenberg S., Remiszewska M.
    The effect of short-term lyophilized beer on established hypertension in rats.
    Food Chem Toxicol 45, 296-302 (2007).
  • Jastrzębski Z., Leontowicz H., Leontowicz M., Namieśnik J., Zachwieja Z., Barton H., Pawelzik E., Arancibia-Avila P., Toledo F., Gorinstein S.
    The bioactivity of processed garlic (Alium sativum) L.) as shown in vitro and in vivo studies on rats.
    Food Chem Toxicol. 45, 1626-1633 (2007).
  • Jastrzębski Z., Napiórkowska L., Pykało R.
    The histopathological evaluation of the local tolerance of alendronate containing medicines.
    Endokrynol Pol 58, 11-17 (2007).
  • Jastrzębski Z., Tashma Z., Katrich El., Gorinstein S.
    Biochemical characteristics of the herb mixture Prolipid as a plant food and medical remedy.
    Plant Foods Human Nutr 62, 145- 150 (2007).
  • Leontowicz M., Leontowicz H., Drzewiecki J., Jastrzebski Z., Haruenkit R., Poovarodom S., Park Y-S., Jung S-T., Kang S-G., Trakhtenberg S., Gorinstein S.
    Two exotic fruits positively affect rat`s plasma composition.
    Food Chem 102, 192-200 (2007).
  • Leontowicz M., Leontowicz H., Jastrzębski Z., Jesion I., Haruenkit R., Poovarodon S., Katrich E., Tashma Z., Drzewiecki J., Trakhtenberg S., Gorinstein S.
    The nutritional and metabolic indices in rats fed cholesterol-containing diets supplemented with durian at different stages of ripening.
    Biofactors 29, 123-136 (2007).
  • Spławiński J i wsp.:
    Pharmacogenetics. Towards improving treatment with medicines.
    W: CIOMS, Genewa 2005 (rozdział w książce).
  • Spławiński J,. Kuźniar J,. Kurianowicz R., Wanczura P.:
    Bioequivalence of two preparations of ticlopidine evaluated using pharmacodynamic end point.
    Int J Clin Pharmacol Therap 2005; 43:452-456.
  • Spławiński, J. Kuźniar, K. Filipiak, W. Zieliński:
    Evaluation of drug toxicity in clinical trials.
    Sci Eng Ethics 2006; 12:139-145