DEPARTMENT OF BIOCHEMISTRY AND BIOPHARMACEUTICALS (BB)

Personnel and structure:

Head of the Department: Elżbieta Anuszewska, Professor
 e-mail: 

Secretary: Joanna Osińska
phone: +48 22 841-21-65
+48 22 841-39-91
phone/fax:  +48 22 841-54-31

Vitamins Lab
Head: Beata Gruber Ass. Prof.
Irena Bubko Ph.D.
Jolanta Krzysztoń-Russjan Ph.D.
Gabriela Kłaczkow M.Sc.
Jarosław Syska M.Sc.
Adrian Woźniak

Biological Lab
Head: Jadwiga Marczewska Ph.D.
Janina Drozd Ph.D.
Ewa Karwicka M. Sc.
Magdalena Mirecka

Biopharmaceutics Lab
Head: Jadwiga Dudkiewicz Wilczyńska Ph.D.
Iza Książek M.Sc.
Karolina Nowak M.Sc.
Agnieszka Grabowska M.Sc.
Magdalena Rosłon M.Sc.

Proteins and Enzymes Lab
Head: Małgorzata Wilk Ph.D.
Małgorzata Jaworska M.Sc.
Marta Stańczyk M. Sc.
Bożena Biczyk techn.

Activities

Quality control of medicinal products:

Statutory control of medicinal products quality, safety and efficacy; evaluation of analytical dossier of the following groups of medicinal products:
  • Vitamins (one compound products or multivitamins with minerals), natural oils, i.e.: cod liver oils and fish oils or plant oils with vitamins, vitamin metabolites and derivatives
  • immunological substances, as interferons, growth factors, monoclonal antibodies
  • blood products
  • natural and synthetic peptides, aminoacids and their derivatives
  • enzymes and their derivatives
  • mukopolisaccharides
  • natural and synthetic protein hormones as insulin, erythropoietin
  • cytostatics (etoposide, cyclophosphamide)
  • synthetic antidiabetic compounds
  • products of animal organs
  • medical devices for diagnostics
  • cyto- and genotoxicity of medical devices
  • food special for medical purposes (FSMP)
  • biopharmaceuticals (products of biotechnology)

The wide spectrum of analytical methods is used in the Department including: spectrophotometry, spectrofluorometry, flame photometry, chromatographic methods (TLC, HPLC), electrophoretic methods (CE, CPS, PAGE, IEF), densitometry, enzymatic methods, and also biological and biochemical tests in vitro and in vivo, RT-PCR, PCR, microarray.

The Department currently uses many tests in vitro based on the bacterial, animal or human cell cultures. These are: Ames test, MTT or EZ4U test, test with neutral red or trypan blue exclusion, micronucleus test, comet assay, SOS-chromotest, tests for immunomodulatoring activity evaluation, tests for detection of anti-HIV-1/2 and antigen HBs

Educational activity:

The Department organizes summer laboratory trainings and supervises Master works for students of Pharmacy Faculty, Medical University of Warsaw and also of the other biotechnological, chemical and biological Faculties.

Research:

Studies on:
  • cytotoxic and genotoxic mechanism of action of anthracyclines - commonly used anticancer drugs
  • increase of antitumour efficacy of antineoplastic drugs when combined with non-steroid antiinflammatory agents
  • relationship between antioxidative enzymes activity in the cells and their sensitivity to anthracyclines
  • influence of DNA repair activity and the specified DNA damages on cytotoxic activity of anthracyclines
  • possible correlation between apoptotic activity of anthracyclines and cells sensitivity to these drugs
  • elaboration of the methods for vitamins determination in pharmaceutical preparations and biological samples using HPLC technique
  • adaptation of chiral liquid chromatography for determination of enantiomeric purity of the tested compounds
  • mechanisms of vitamins action
  • searching for the new medicines from the group of inhibitors of angiotensin-converting enzymes and renin inhibitors - evaluation of their activity in vitro
  • practical use of capillary electrophoresis for pharmaceutical analysis
  • organic and biosynthetic glycoproteins used in therapy
  • analysis of gene expression profiles induced by xenobiotics in the chosen cell lines with use of DNA microarray and RT-PCR
  • immunological activity of pharmacopoeial herbal medicinal products
  • antitumour and antiangiogenic activity of the mutated forms of plasminogen-activator type-1 inhibitor PAI-1

Projects

Scientists of the Department currently participate in 1 research project:

The mutated forms of plasminogen-activator type-1 inhibitor PAI-1 as the proteins with antitumour activity - analysis of farmakokinetics and biological activity towards neoplastic cells and vascular endothelium. Dec. No. 3839/P01/2006/31 z dn. 15.09.2006 r.

Cooperation:

  • Department of Physiology, Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Head Tomasz Motyl, Professor
  • Department of Organic Chemistry, Medical University of Warsaw, Head Dorota Maciejewska, Professor
  • Laboratory of Molecular Diagnostics and Immunology, Institute of Tuberculosis and Lung Disease, Warsaw, Head Joanna Chorostowska-Wynimko, Ass. Professor
  • Medical University of Poznań, Social Medicine, Head Jerzy T. Marcinkowski, Professor
  • The European Directorate for the Quality of Medicines (EDQM)
  • Department of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Warsaw, Head Olga Olszowska, Professor
  • Interdepartment Biotechnological Studies, Warsaw University of Life Sciences (SGGW)
  • Faculty of Chemistry, Warsaw University of Technology, Head A.R. Kunicki, Professor
  • Center of Bone Marrow Transplantation, CSK MON WIM, Warsaw, Head Piotr Rzepecki, Professor

Services:

  • evaluation of analytical methods for determination of pharmaceutical substances and their impurities, and validation of the methods
  • determination of enzymatic activity (including microplate methods), especially of the pancreatic enzymes and pepsin
  • evaluation of mutagenicity (Ames test) using bacteria Salmonella typhimurium (TA 97, TA 98, TA 100, TA 102)
  • evaluation of genotoxicity using micronucleus test in vitro, SOS-chromotest, comet assay
  • evaluation of cytotoxicity using MTT or EZ4U test, tests with neutral red or trypan blue exclusion
  • evaluation of cytotoxicity and genotoxicity of medical devices according to ISO 10993
  • evaluation of immunomodulating effects using rosette-forming test, hemagglutination test and cytotoxicity test
  • HBs antigen and HIV 1/2 antibody assay

Equipment:

  • HPLC system Shimadzu (5) and Dionex (2) with UV/VIS, DAD, RF and RI detectors
  • spectrophotometer UV/VIS (´3)
  • microplate spectrophotometer
  • spectrofluorometer Jasco
  • capillary electrophoresis Qarta 4000
  • electrophoresis system Paragon Appraise
  • the sets of horizontal and vertical gel electrophoresis
  • flame photometer C-410
  • TLC densitometer
  • TLC sampler applicator III
  • dissolution test system PTW II
  • disintegration test instrument
  • freezer (-70 oC)
  • autoclave
  • cabinet with laminar air-flow (´5)
  • incubator (´5)
  • thermoblock TB-941T
  • microscope Nikon ECLIPSE TS100 equipped with camera
  • cell coulter Z2
  • fraction collector Gilson
  • system for Image Analysis BioRad
  • system for Image Processing and Analysis Lucia G Komet
  • system for Real Time-PCR and PCR analysis
  • system for quick electrophoresis Lonza-FlashGel System

Main publications since 2004

  1. B.M. Gruber, E.L. Anuszewska, I. Bubko, A. Goździk, I. Fokt, W. Priebe.
    Effect of structural modification at the 4, 3' and 2' positions of doxorubicin on topoisomerase II poisoning, apoptosis and cytotoxicity in human melanoma cells.
    Arch. Immunol. Ther. Exp., 2007, 55: 193-196. .
  2. E. Karwicka, J. Marczewska, E. Anuszewska, B. Łozowicka, Z. Chilmonczyk
    Genotoxicity of α-asarone and its analogues.
    Bioorganic & Medicinal Chemistry, 2008, 16: 6069-6074.
  3. J.Chorostowska-Wynimko, M.Kędzior, R.Struniawski, B.Gruber, E.Anuszewska, A.Aleem, J.Jankun
    The MTT assay is not adequate for assessment of mutated PAI-1 toxicity towards endothelial cells.
    Central Eur. J. Immunol. 33(4), 182-184, 2008
  4. G.Kłaczkow, E.Anuszewska
    Determination of stereoisomers of racemic α-tocopherol in pharmaceutical preparation by high-performance liquid chromatography and gas chromatography
    Acta Polon. Pharm.-Drug Res. 65(6), 715-721, 2008
  5. J.Krzysztoń-Russjan, I.Książek, E.Anuszewska
    Porównanie użyteczności testów MTT i EZ4U stosowanych do oceny cytotoksyczności ksenobiotyków
    Farm. Pol. 65(6), 395-402, 2009
  6. M.Jaworska, P.Cygan, M.Wilk, E.Anuszewska
    Capillary elektrophoresis with indirect UV detection for the determination of stabilizers and citrates present in human albumin solutions
    J. Pharm. Biomed. An. 50, 90-95, 2009
  7. J. Chorostowska -Wynimko, M. Kędzior, R. Struniawski, B. Gruber, E. Anuszewska, E. Skrzypczak-Jankun, J. Jankun
    Mutated PAI-1 inhibits proliferative activity of lung cancer cells.
    J. Thoracic Onkol., 4(9), S 609, 2009
  8. J. Dudkiewicz-Wilczyńska, K. Nowak, E. Anuszewska
    Jakość odtwórczych produktów diabetologicznych. Porównanie metod stosowanych do oceny zanieczyszczeń w produktach z glimepirydem.
    Farm. Pol. 65(9), 1-5, 2009
  9. I. Winiecka, J. Dudkiewicz-Wilczyńska, I. Roman, R. Paruszewski
    New potential renin inhibitors with dipeptide replacements in the molecule.
    Acta Polon. Pharm.-Drug Res. 67(4), 367-374, 2010
  10. J.Drozd, E.Anuszewska
    Effects of bilberry fruit aqueous extract and selected antibiotics on immune response in mice
    Acta Polon. Pharm.-Drug Res. 66(2), 181-184, 2009
  11. B.M. Gruber, I. Bubko, J. Krzysztoń-Russjan, E.L. Anuszewska
    Synergistic action od doxorubicin and sulindac in human cervix carcinoma cells - studies on the possible mechanisms.
    Med. Sci Monit., 16(1), BR45-51, 2010